In front of nearly 82,000 fans Nani had put United ahead in the 22nd minute.
Barcelona, without Lionel Messi equalized in the 70th minute with a stunning strike from Thiago Alcantara.
Owen latched on to graduate from Tom Cleverley scored United's winning goal during their final tour of North America won five wins from five.
Owen tweeted after the match: "As normal with Barca striker got a touch difficult opponent's case Luckily I scored a goal but I missed the night nanny too!."
United beaten by Barcelona in the Champions League final at Wembley in May, and a record crowd for a football match in the capital of United Premier League champions look to get the small size of revenge.
Sir Alex Ferguson's side will return home and prepare for the Community Shield against Manchester City won the FA Cup at Wembley next Sunday.
Ferguson's assistant manager Mike Phelan said: "Tonight is not do-or-die situation it is important that the players we put out in the field understand what it takes to play against a great team ..
"We have some very good young players out there and [they] are proud to own a high standard."
Wayne Rooney missed an early chance before the opening goal Nani was created by Danny Welbeck on the counterattack. Portugal winger beat the offside trap and scored with a leg Barcelona goalkeeper Victor Valdes.
Messi missing and chose not to play Barcelona Xavi, Carlos Puyol or Gerard Pique in their starting line-up. Pep Guardiola's team draw level with 20 minutes remaining when 20-year midfielder Thiago scored with a curved incredible new flying past the United goalkeeper David De Gea.
The game will go to penalties if it completed the basic level, but Owen ensure that the American left North America with 100% winning record when he cracked the ball past Valdes following some sloppy play defensively from Barcelona.
Brazilian defender Rafael injured in the first half and was replaced by his brother Fabio.
Ferguson confirmed after the match that Mexico striker Javier Hernandez was given the all clear to fly back with the squad after he suffered a concussion in training sessions.
"Javier will come back with us he's fine .. He will not play for several weeks but it has also occurred.
"He's always going to need a rest until he regains fitness he will do some training, then we would have come back in three to four weeks .."
Ferguson also dismissed speculation about the club's ongoing approach to sign Dutch international Wesley Sneijder from Inter Milan, said: "Absolute nonsense I never talk about him there is nothing new ..."
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